Invited Speaker



Update time:2024-01-02 20:44



     从事干细胞与骨发育、再生医学研究20余年。在Nat Med、Bioact Mater、Dev Cell、PNAS、Biodes Manuf等刊物发表骨科研究论文30余篇,引用率高。2014年全职回国,组建骨发育与再生科研团队,现有固定成员8人,博士及硕士研究生19人。主持国家自然科学基金-广东联合基金重点项目1项、面上项目2项,重庆市基础研究重点项目1项等。前期成果入选美国骨矿盐疾病研究学会(ASBMR)2013年度杰出研究,文章被《Nature Reviews》系列杂志连续专题报道。


Topic title: Bioprinting metabolic and neurovascularized bone organoid for regenerative repair


The artificial reconstruction of simulated bones with dual functions of mechanical support and authentic bioactivities, including bone formation and resorption, remains a huge challenge for orthopedic regenerative medicine. Scientists have successfully used functional cells or growth factors to simulate the microenvironment of tissue/organ development, guiding stem cell differentiation and self-assembly to construct organoids (Nat Rev Mater 2019, 4:606). Among these organoids, the optical cup has been used in the clinical treatment of macular lesions to restore patients’ vision (Trends Mol Med 2022, 28:388). Therefore, in this regard, building authentic skeleton requires physiological osteogenic microenvironments (POME).

In 2016, an integrated tissue-organ printer was able to biologically generate structures of the supporting hard materials and stem cells with clinically relevant size, shape, and structural integrity, which were implanted into murine skulls to form vascularized bone (Nat Biotechnol 2016, 34:312). This work is considered as a milestone of orthopedic medicine and opens up a novel avenue for future medical applications. However, seven years later, this study did not make further breakthroughs in bone reconstruction, possibly due to a lack of application in the POME (Chem Rev 2020, 120:11056).

Here, we constructed a bone developmental module (BDM) using an ourself-developed hard material and cell integrated 3D bioprinting system (Biodes Manuf 2022, 5:487). This module utilizes supporting materials with improved physical and chemical properties, biocompatibility, and nutrient and metabolite channels for cell growth, as well as a POME we discovered (PNAS 2015, 112:e478). The POME is the osteocyte with dominant active Wnt/β-catenin signaling, called daCOOME. The elastic modulus of the module is 11.3 MPa, and the calcium:phosphorus ratio is 1.5, reaching the strength and biocompatibility of murine cancellous bone. Additionally, the hydrogel loaded with cells is consolidated by double crosslinking in BDM, and the channels between the hydrogels are prefabricated by sacrificial material Pluronic F-127, which ensures that the cells grow ex vivo for up to 28 days, and the cell survival rate in the first 7 days is as high as 92%. daCOOME promotes cell proliferation, osteogenic differentiation and mineralization of bone marrow stromal cells, and produces VEGF and NGF, recruiting HUVEC and nerval progenitor cells for angiogenesis and neurogenesis. In vivo, the BDM forms a bone organoid, containing osteoblasts, osteoclasts, H-type blood vessels, and the cells that express neural markers, such as β3-tubulin and NeuN as authentic bone develops. The BDM conforms to bone mechanics and accelerates the repair of critical-sized defects of parietal bone, providing a promising strategy for orthopedic regenerative medicine.

Congress has ended
Important Dates
Conference Dates
March 29-31, 2024
Deadline for Submission of Abstract

December 31, 2023

Still open for submission

Notification of Abstract Acceptance

January 15, 2024

January 25, 2024