Invited Speaker



Update time:2024-01-30 12:50


Topic title: 3D Printed Biomimetic Scaffold for Integrative Osteochondral Regeneration


The integrative regeneration of both articular cartilage and subchondral bone remains an unmet clinical need due to the difficulties of mimicking spatial complexity in native osteochondral tissues for artificial implants. With the layer-by-layer fabrication strategies, 3D printing becomes a promising technology to replicate the stratified zonal architecture and vary micro-structures and mechanical properties. However, the dynamic and circulating physiological environments, such as mass transportation or cell migration, usually distort the pre-confined biological properties in the layered implants, which leads to undistinguished spatial variations and subsequently inefficient regenerations. Here, we introduce a biomimetic calcified interfacial layer into the scaffold as a compact barrier between a cartilage layer and a subchondral bone layer to facilitate osteogenic-chondrogenic repair. The calcified interfacial layer consisting of compact polycaprolactone (PCL), nano-hydroxyapatite (nHA), and tasquinimod (TA) can physically and biologically separate the cartilage layer (TA-mixed, chondrocytes-load gelatin methacrylate) from the subchondral bond layer (porous PCL). The introduction of the calcified interfacial layer preserves the as-designed independent biological environment in each layer for both cartilage and bone regeneration, successfully inhibits vascular invasion into the cartilage layer and prevents hyaluronic cartilage calcification. The improved integrative regeneration of cartilage and subchondral bone have been validated via gross examination, micro-computed tomography (micro-CT), and histological and immunohistochemical analysis based on in vivo rat model. Moreover, the gene and protein expression studies identify a key role of CAV-1 to promote angiogenesis by the Wnt/β-catenin pathway, and indicate that TA in the calcified layer block angiogenesis through inhibiting CAV-1.

Congress has ended
Important Dates
Conference Dates
March 29-31, 2024
Deadline for Submission of Abstract

December 31, 2023

Still open for submission

Notification of Abstract Acceptance

January 15, 2024

January 25, 2024