阿力木•克热木，博士，主任医师，硕士研究生导师。喀什地区第一人民医院院长助理、创伤骨科主任。中国医师协会显微外科医师分会第二届委员会委员、中国医师协会显微外科医师分会第二届委员会数字与智能显微外科组副主任委员、新疆医学会骨科分会副主任委员（青年）、新疆创伤救治联盟理事会副理事长、新疆医学会创伤外科与急救分会常务委员、喀什地区医学会骨科专业委员会主任委员、“中华显微外科杂志”第十一届编辑委员会编辑委员、《中华创伤杂志》、《Orthopedics》审稿人。

主持自治区科技重大项目子课题“全疆区域数字骨科中心构建及应用”、主持省部级创新团队项目“数字骨科创新团队”、参与国家重点研发项目“骨科机器人在边远地区的应用示范”等科研项目12项。第一作者及通讯作者发表学术论文30余篇，其中SCI论文12篇。荣获全国卫生系统青年岗位能手、喀什青年五四奖章、自治区天山英才（第三期），第一届、第二届全疆医护技能大赛团体冠军等荣誉。

Keremu·Alimu

Academic position: Member of the second Committee of Microsurgery Branch of Chinese Medical Doctor Association, Deputy Chairman of Digital and intelligent microsurgery Group of the second Committee of Microsurgery Branch of Chinese Medical Doctor Association, Deputy chairman (youth) of Orthopaedic Branch of Xinjiang Medical Association, Deputy chairman of the Council of Xinjiang Trauma Treatment Alliance, standing member of Trauma Surgery and First Aid Branch of Xinjiang Medical Association, Kashgar Regional Medical Association Chairman of Orthopedics Professional Committee, editorial member of the 11th Editorial Committee of Chinese Journal of Microsurgery, reviewer of Chinese Journal of Trauma and Orthopedics.

Presided over the sub-project of the autonomous region's major science and technology project "Construction and application of digital orthopedics center in Xinjiang", presided over the provincial and ministerial innovation team project "digital orthopedics innovation team", participated in the national key research and development project "Orthopedics robot application demonstration in remote areas" and other 12 scientific research projects. The first author and corresponding author have published more than 30 academic papers, including 12 SCI papers. He won the national Health system youth post expert, Kashgar Youth May Fourth Medal, Tianshan Talents of the Autonomous Region (the third period).

Topic title: 3D printed PLGA scaffold with nano-hydroxyapatite carrying linezolid for treatment of infected bone defects

Abstract:

Background: Linezolid has been reported to protect against chronic bone and joint infection. In this study, linezolid was loaded into the 3D printed poly (lactic-co-glycolic acid) (PLGA) scaffold with nano-hydroxyapatite (HA) to explore the effect of this composite scaffold on infected bone defect (IBD). Methods: PLGA scaffolds were produced using the 3D printing method. Drug release of linezolid was analyzed by elution and high-performance liquid chromatography assay. PLGA, PLGA-HA, and linezolid-loaded PLGA-HA scaffolds, were implanted into the defect site of a rabbit radius defect model. Micro-CT, H&E, and Masson staining, and immunohistochemistry were performed to analyze bone infection and bone healing. Evaluation of viable bacteria was performed. The cytocompatibility of 3D-printed composite scaffolds in vitro was detected using human bone marrow mesenchymal stem cells (BMSCs). Long-term safety of the scaffolds in rabbits was evaluated. Results: The linezolid-loaded PLGA-HA scaffolds exhibited a sustained release of linezolid and showed significant antibacterial effects. In the IBD rabbit models implanted with the scaffolds, the linezolid-loaded PLGA-HA scaffolds promoted bone healing and attenuated bone infection. The PLGA-HA scaffolds carrying linezolid upregulated the expression of osteogenic genes including collagen I, runt-related transcription factor 2, and osteocalcin. The linezolid-loaded PLGA-HA scaffolds promoted the proliferation and osteogenesis of BMSCs in vitro via the PI3K/AKT pathway. Moreover, the rabbits implanted with the linezolid-loaded scaffolds showed normal biochemical profiles and normal histology, which suggested the safety of the linezolid-loaded scaffolds. Conclusion: Overall, the linezolid-loaded PLGA-HA scaffolds fabricated by 3D printing exerts significant bone repair and anti-infection effects.